Specific Objectives

  1. To develop specific guidelines for monitorization of antibiotics and AMR in environmental samples through a combined chemical and biological (phenotypic, genotypic and metagenomic) approach.
  2. To establish a biobank of AMR strains isolated from environmental samples that will be freely available for future research.
  3. To establish an early-stage database of ARG sequences and genetic biomarkers for an accurate identification of environmental AMR resistant strains and associated resistance genes.
  4. To map the antibiotic content and antimicrobial resistance patterns in different environments from Cluj county, Romania.
  5. To test the efficiency of nanotechnology (carbon nanotubes) for remowal of antibiotics and AMR microorganisms from wastewaters.
  6. To set up a research infrastructure and a functional bioinformatic center for future state-of-the-art environmental AMR studies using genotypic and metagenomic approaches.

The project proposes a state-of-the-art experimental step by step guideline currently missing in Romania and, from our knowledge, the EU. Using a comprehensive approach that combines several chemical and biological methods EnviroAMR will provide valuable data that will raise awareness on the pollution with antibiotics and the AMR phenomenon in the environment with direct consequences on the quality of surface waters and groundwater. It will provide decision making instruments adressing the Surveillance and Environment priority topics defined by the Joint Programming Initiative on Antimicrobial Resistance (JPIAMR) within the European Union and the main objectives stated in the Romania–World Health Organization (WHO) collaboration that refer to conducting a comprehensive situation assessment on emergence of resistance, as well as establishing a national mechanism and developing action plans for containment of antibiotic and antibiotic resistance. By creating: (i) a molecular biology infrastructure focused on AMR surveillance; (ii) a bioinformatic center for metagenomic studies; (iii) specific methods of investigation and by (iv) the hands-on training for the methods proposed, the project will provide the tools and the knowledge for rapid and modern monitoring of antibiotics, AMR organisms and ARGs in the environment, fulfilling the general objective of the financiing scheme, that is “preventing damage and side effects […] caused by chemicals and hazardous substances”.

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